![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K2100033:Design
Designed by: Kathleen Brandes Group: iGEM16_MIT (2016-10-17)
pEXPR pERE3:TALER14
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190
Illegal EcoRI site found at 212
Illegal EcoRI site found at 386
Illegal XbaI site found at 363 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190
Illegal EcoRI site found at 212
Illegal EcoRI site found at 386 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190
Illegal EcoRI site found at 212
Illegal EcoRI site found at 386
Illegal BamHI site found at 509
Illegal XhoI site found at 452 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190
Illegal EcoRI site found at 212
Illegal EcoRI site found at 386
Illegal XbaI site found at 363 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 180
Illegal EcoRI site found at 190
Illegal EcoRI site found at 212
Illegal EcoRI site found at 386
Illegal XbaI site found at 363 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 524
Design Notes
This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
Source
This is a synthetic promoter with a gene from the mammalian genome.