DNA

Part:BBa_K2100033:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-17)


pEXPR pERE3:TALER14


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 212
    Illegal EcoRI site found at 386
    Illegal XbaI site found at 363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 212
    Illegal EcoRI site found at 386
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 212
    Illegal EcoRI site found at 386
    Illegal BamHI site found at 509
    Illegal XhoI site found at 452
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 212
    Illegal EcoRI site found at 386
    Illegal XbaI site found at 363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 180
    Illegal EcoRI site found at 190
    Illegal EcoRI site found at 212
    Illegal EcoRI site found at 386
    Illegal XbaI site found at 363
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 524


Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This is a synthetic promoter with a gene from the mammalian genome.

References